THE MOLECULAR SIZE AND SHAPE OF THE PANCREATIC PROTEASES I. SEDIMENTATION STUDIES ON CHYMOTRYPSINOGEN AND ON a- AND %CHYMOTRYPSIN
نویسنده
چکیده
Although trypsin, a-, /3-, and y-chymotrypsin, and their inactive precursors were obtained in crystalline form by Kunitz and Northrop (1) and by Kunitz (2) more than 10 years ago, detailed molecular-kinetic characterization of these substances is lacking. The present experiments have been undertaken as part of a comprehensive investigation of the proteolytic enzymes of the pancreas which will include a physicochemical study of the reversible denaturation of some of these proteins. This paper constitutes the first part of a project which will include characterization of these proteins by means of sedimentation, diffusion, and viscometric measurements.
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